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af1997 mouse anti nobox  (R&D Systems)


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    R&D Systems af1997 mouse anti nobox
    Af1997 Mouse Anti Nobox, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 465 article reviews
    af1997 mouse anti nobox - by Bioz Stars, 2026-03
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    Resveratrol-βcd can inhibit the progression of POI in a mouse model. (A) Schematic diagram of resveratrol-βcd treatment; (B) Changes in body weight of the control group, negative control group (resveratrol-βcd alone), POI model group, and treatment group (POI + resveratrol-βcd) during the treatment, n = 5; (C) On the 20 day after treatment began, the ovaries of the mice were isolated and weighed (D) , with each point representing the weight of one ovary, n = 8; (E) Typical images of follicles at different stages in the ovaries, scale bar = 500 μm and the corresponding statistical analysis results (F) , n = 5; (G) Typical western blot result <t>of</t> <t>LHX8</t> and <t>NOBOX</t> expression levels in ovarian tissue, n = 3, see the other two western blot replicates in Supplementary Fig. ; All the repetitive results of western blot in this paper can be found in Supplementary Fig. , which will not be described afterwards; (H) Total RNA was extracted from ovarian tissue, and qPCR was used to analyze the transcriptional changes of Ddx4 and Pou5f1 , n = 5; (I) On day 20 post treatment, mouse serum was collected to detect AMH, FSH and E2 (J) levels in the blood, n = 5. (K) Estrous cycles examination by vaginal smears, scale bar = 200 μm, percentage of time on cycle phase was shown on the right, M/D = Metestrus and Diestrus, n = 10; (L) Reproductive activity analysis of different treatment group, n = 15; Statistical analysis was performed using two-way ANOVA (B) , and one-way ANOVA ( D,F,H,I,J and K ) comparison was made POI vs. control, POI vs. resveratrol-βcd and POI vs. POI + resveratrol-βcd; ns, not significant
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    Resveratrol-βcd can inhibit the progression of POI in a mouse model. (A) Schematic diagram of resveratrol-βcd treatment; (B) Changes in body weight of the control group, negative control group (resveratrol-βcd alone), POI model group, and treatment group (POI + resveratrol-βcd) during the treatment, n = 5; (C) On the 20 day after treatment began, the ovaries of the mice were isolated and weighed (D) , with each point representing the weight of one ovary, n = 8; (E) Typical images of follicles at different stages in the ovaries, scale bar = 500 μm and the corresponding statistical analysis results (F) , n = 5; (G) Typical western blot result <t>of</t> <t>LHX8</t> and <t>NOBOX</t> expression levels in ovarian tissue, n = 3, see the other two western blot replicates in Supplementary Fig. ; All the repetitive results of western blot in this paper can be found in Supplementary Fig. , which will not be described afterwards; (H) Total RNA was extracted from ovarian tissue, and qPCR was used to analyze the transcriptional changes of Ddx4 and Pou5f1 , n = 5; (I) On day 20 post treatment, mouse serum was collected to detect AMH, FSH and E2 (J) levels in the blood, n = 5. (K) Estrous cycles examination by vaginal smears, scale bar = 200 μm, percentage of time on cycle phase was shown on the right, M/D = Metestrus and Diestrus, n = 10; (L) Reproductive activity analysis of different treatment group, n = 15; Statistical analysis was performed using two-way ANOVA (B) , and one-way ANOVA ( D,F,H,I,J and K ) comparison was made POI vs. control, POI vs. resveratrol-βcd and POI vs. POI + resveratrol-βcd; ns, not significant
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    Image Search Results


    Resveratrol-βcd can inhibit the progression of POI in a mouse model. (A) Schematic diagram of resveratrol-βcd treatment; (B) Changes in body weight of the control group, negative control group (resveratrol-βcd alone), POI model group, and treatment group (POI + resveratrol-βcd) during the treatment, n = 5; (C) On the 20 day after treatment began, the ovaries of the mice were isolated and weighed (D) , with each point representing the weight of one ovary, n = 8; (E) Typical images of follicles at different stages in the ovaries, scale bar = 500 μm and the corresponding statistical analysis results (F) , n = 5; (G) Typical western blot result of LHX8 and NOBOX expression levels in ovarian tissue, n = 3, see the other two western blot replicates in Supplementary Fig. ; All the repetitive results of western blot in this paper can be found in Supplementary Fig. , which will not be described afterwards; (H) Total RNA was extracted from ovarian tissue, and qPCR was used to analyze the transcriptional changes of Ddx4 and Pou5f1 , n = 5; (I) On day 20 post treatment, mouse serum was collected to detect AMH, FSH and E2 (J) levels in the blood, n = 5. (K) Estrous cycles examination by vaginal smears, scale bar = 200 μm, percentage of time on cycle phase was shown on the right, M/D = Metestrus and Diestrus, n = 10; (L) Reproductive activity analysis of different treatment group, n = 15; Statistical analysis was performed using two-way ANOVA (B) , and one-way ANOVA ( D,F,H,I,J and K ) comparison was made POI vs. control, POI vs. resveratrol-βcd and POI vs. POI + resveratrol-βcd; ns, not significant

    Journal: Journal of Ovarian Research

    Article Title: Resveratrol-βcd inhibited premature ovarian insufficiency progression by regulating granulosa cell autophagy

    doi: 10.1186/s13048-024-01344-0

    Figure Lengend Snippet: Resveratrol-βcd can inhibit the progression of POI in a mouse model. (A) Schematic diagram of resveratrol-βcd treatment; (B) Changes in body weight of the control group, negative control group (resveratrol-βcd alone), POI model group, and treatment group (POI + resveratrol-βcd) during the treatment, n = 5; (C) On the 20 day after treatment began, the ovaries of the mice were isolated and weighed (D) , with each point representing the weight of one ovary, n = 8; (E) Typical images of follicles at different stages in the ovaries, scale bar = 500 μm and the corresponding statistical analysis results (F) , n = 5; (G) Typical western blot result of LHX8 and NOBOX expression levels in ovarian tissue, n = 3, see the other two western blot replicates in Supplementary Fig. ; All the repetitive results of western blot in this paper can be found in Supplementary Fig. , which will not be described afterwards; (H) Total RNA was extracted from ovarian tissue, and qPCR was used to analyze the transcriptional changes of Ddx4 and Pou5f1 , n = 5; (I) On day 20 post treatment, mouse serum was collected to detect AMH, FSH and E2 (J) levels in the blood, n = 5. (K) Estrous cycles examination by vaginal smears, scale bar = 200 μm, percentage of time on cycle phase was shown on the right, M/D = Metestrus and Diestrus, n = 10; (L) Reproductive activity analysis of different treatment group, n = 15; Statistical analysis was performed using two-way ANOVA (B) , and one-way ANOVA ( D,F,H,I,J and K ) comparison was made POI vs. control, POI vs. resveratrol-βcd and POI vs. POI + resveratrol-βcd; ns, not significant

    Article Snippet: The primary antibodies used in this experiment included LHX8 (Sigma, SAB2101342), NOBOX (Sigma, SAB2105362), SQSTM1 (CST, #88,588), LC3I/II (LC3A/B CST, #12,741), β-actin (Proteintech, 20536-1-AP), JAK2 (CST, #3230), and p-JAK2 (CST, #3771).

    Techniques: Negative Control, Isolation, Western Blot, Expressing, Activity Assay, Comparison